[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 10, Issue 3 (autumn 2010) ::
J Ardabil Univ Med Sci 2010, 10(3): 223-231 Back to browse issues page
Determination of cytokine profile following the immunization with Brucella abortus S99 lipopolysaccharide_Neisseria meningitidis serogroup B outer membrane vesicle complex in animal model
Seyed Davar Siadat , Hoda Shirdast, Mohammadreza Aghasadeghi, Darioush Norouzian, Seyed Mohammad Atyabi, Seyed Mahdi Sadat, Sanaz Soleymaniyeh, Ali Sharifat, Arfa Moshiri
Abstract:   (5408 Views)

  Background & Objectives: Lipopolysaccharide (LPS) is the main antigenic structure expressed on the surface of smooth strains of Brucella. It has been shown that Outer membrane vesicle (OMV) of Neisseria meningitidis efficiently promote IgG and IgM response against the administrated antigen as an adjuvant. The aim of this study is to evaluate the effect of LPS-OMV noncovalent complex in producing of T helper 1 cytokine (IFN-γ) and T helper 2 cytokines (IL-4 and IL-10) in mice.

  Methods: LPS extracted by an optimized method based on hot phenol-water extraction. Groups of six BALB/c mice were injected subcutaneously with LPS alone, LPS with Freund adjuvant and LPS-OMV complex on 0, 14 and 28 days. Levels of IFN-γ, IL-4 and IL-10 were evaluated in spleen cell suspension supernatant by ELISA.

  Result: Immunization with B. abortus LPS significantly induced high level of IFN-γ in comparison to the other groups immunized with LPS-OMV and LPS+ adjuvant (p<0.05). In contrast, lower levels of IL-4 and IL-10 were elicited by LPS in the rest groups. Immunization with the non-covalent complex of B. abortus LPS-N. meningitidis serogroup B OMV caused a significant increase of IL-4 and IL-10 compared with the mice immunized with B. abortus LPS (p<0.05), while the titer of IFN-γ is still significantly higher than IL-4 and IL-10 (p<0.05).

  Conclusion: The raise of IFN-γ following the immunization with all of the compounds (LPS, LPS-OMV non-covalent complex and LPS+adjuvant) indicates the activation of Th1 population that would be correlated to the clearance of the organism due to the amplification of anti-microbial activity of Polymorphonuclear cells. Low levels of IL-4 and IL-10 following the immunization with all compounds would be a sign of Th1 responses dominancy or inhibition of Th2 population proliferation and activity. Such a cytokine pattern would be a sign of the efficiency of brucellosis subunit vaccine because Th2 responses basically have no role in the immune responses against Brucella and may lead to the persistence of intracellular infection.

Keywords: Brucella abortus; Lipopolysaccharide; Neisseria meningitidis; Outer membrane vesicle; IFN-γ; IL-10; IL-4
Full-Text [PDF 391 kb]   (1524 Downloads)    
Type of Study: article | Subject: Special
Received: 2010/09/20 | Accepted: 2010/12/9 | Published: 2011/01/8
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Siadat S D, Shirdast H, Aghasadeghi M, Norouzian D, Atyabi S M, Sadat S M, et al . Determination of cytokine profile following the immunization with Brucella abortus S99 lipopolysaccharide_Neisseria meningitidis serogroup B outer membrane vesicle complex in animal model. J Ardabil Univ Med Sci. 2010; 10 (3) :223-231
URL: http://jarums.arums.ac.ir/article-1-213-en.html


Volume 10, Issue 3 (autumn 2010) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
Persian site map - English site map - Created in 0.1 seconds with 32 queries by YEKTAWEB 3986