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:: Volume 16, Issue 4 (winter 2017) ::
J Ardabil Univ Med Sci 2017, 16(4): 353-362 Back to browse issues page
Investigation on Ornithine Decarboxylase Activity and Level of Putrescine, Spermidine and Spermine in Hydatid Cyst
Somayeh Bahrami , Hossein Najafzadeh, Ali Shahriari, Sara Ahmadi, Mohammadhossein Razi Jalali
Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (6154 Views)

Background & objectives: Polyamines such as putrescine, spermidine, and spermine are ubiquitous in all eukaryotic cells and play an essential role in cell division and differentiation. One way of polyamine biosynthesis is done by ornithine decarboxylase (ODC) which catalyzes the transformation of ornithine to putrescine. The aim of the present study was to evaluate the level of putrescine, spermidine and spermine in protoscolices, hydatid fluid and germinal layer and also to evaluate ODC activity.

Methods: In the present study putrescine, spermidine and spermine levels were investigated in germinal layers, hydatid fluids and protoscolices. To evaluate the activity of ODC, protoscolices were incubated with ornithine and changes in polyamines level were assayed. The samples were homogenized and liquid chromatography (HPLC) was used for polyamines measurement.

Results: Based on the results, putrescine was the lowest polyamine and since its level was not increased in protoscolices incubated with ornithine, ODC activity was not detected. Spermidine was the highest polyamine and the results showed that germinal layer contained the highest level of polyamines.

Conclusion: Overall, the results showed that ODC activity was not detected in hydatid cyst and level of polyamines in germinal layers  which contained rapidly proliferating cells was higher than other parts.

Keywords: Hydatid cyst, Polyamines, Ornithine Decarboxylase
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Type of Study: article | Subject: General
Received: 2016/03/10 | Accepted: 2016/09/26 | Published: 2016/12/30
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Bahrami S, Najafzadeh H, Shahriari A, Ahmadi S, Razi Jalali M. Investigation on Ornithine Decarboxylase Activity and Level of Putrescine, Spermidine and Spermine in Hydatid Cyst. J Ardabil Univ Med Sci. 2017; 16 (4) :353-362
URL: http://jarums.arums.ac.ir/article-1-1245-en.html


Volume 16, Issue 4 (winter 2017) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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